Novel group within the kingdom Crenarchaeota from boreal forest soil.
نویسندگان
چکیده
We report here the results of phylogenetic analysis of archaeal 16S rRNA gene sequences amplified by PCR with Archaea-specific primers with mixed-population DNA extracted directly from forest soil used as a template. Nucleotide signature and phylogenetic analyses show that the sequences obtained belong to the domain Archaea and form a new cluster. Its phylogenetic position suggests that sequences are from a previously undescribed terrestrial group within the kingdom Crenarchaeota.
منابع مشابه
Bomberg and Timonen 1 Title ; Effect of tree species and mycorrhizal colonisation on the archaeal population of boreal forest
8 Group 1.1c Crenarchaeota are the predominating archaeal group in acidic boreal forest soils. In this 9 study we show that the detection frequency of 1.1c crenarchaeotal 16S rRNA genes increased in the 10 rhizospheres of the boreal forest trees following colonisation by the ectomycorrhizal fungus Paxillus 11 involutus. This effect was very clear in the fine roots of Pinus sylvestris, Picea abi...
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Front cover: "Archaea's Universe"-collage made by author. Background: combination of same field micrographs made with epifluorescence microscope using filter sets specific for DAPI (blue color) and CY3 (red color, euryarchaeota specific probe EURY499, paper IV), Valkea Kotinen Lake water sample. Center: 16S rRNA secondary structure diagram of the archaeal soil clone FFSB6-one of the first "non-...
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متن کاملHigh abundance of Crenarchaeota in a temperate acidic forest soil.
The objective of the study was to elucidate the depth distribution and community composition of Archaea in a temperate acidic forest soil. Numbers of Archaea and Bacteria were measured in the upper 18 cm of the soil, and soil cores were sampled on two separate occasions using quantitative PCR targeting 16S rRNA genes. Maximum numbers of Archaea were 0.6-3.8 x 10(8) 16S rRNA genes per gram of dr...
متن کاملSoil pH regulates the abundance and diversity of Group 1.1c Crenarchaeota.
Archaeal communities in many acidic forest soil systems are dominated by a distinct crenarchaeal lineage Group 1.1c. In addition, they are found consistently in other acidic soils including grassland pasture, moorland and alpine soils. To determine whether soil pH is a major factor in determining their presence and abundance, Group 1.1c community size and composition were investigated across a ...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 63 2 شماره
صفحات -
تاریخ انتشار 1997